Last data update: 24 January 2024 16:39 CET
Plasmid name: pEXPR-myr-APEX (LMBP 9554)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9554.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Glycine max cytosolic ascorbate peroxidase 1 cDNA (APX1, GeneID 553156); monomeric enhanced mutant (APEX) Mouse lymphocyte protein tyrosine kinase cDNA (Lck); myristoylation-targeting sequence, N-terminal Homo sapiens hypothetical LOC81854 cDNA; stuffer fragment |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pdcDNA-R4R3-dhCMV; pENTR-L4R1-hCMV-myr; pENTR223-MGC3771; pENTR-soybeanAPEX |
| Further information: | This Gateway expression vector was constructed via multisite Gateway reaction between pdcDNA-R4R3-dhCMV, pENTR-L4R1-hCMV-myr, pENTR223-MGC3771 and pENTR-soybeanAPEX. APEX, the monomeric enhanced APX mutant, contains the three engineered mutations K41D, W41F and E112K and is used as a reporter to generate contrast for electron microscopy and also for proteomic tagging in living cells. Other name of the plasmid is pEXPR_myr_APEX. |
| EMBL Accession number: | - |
| Latest sequence update: | 20/03/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. De Ceuleneer(1) and Prof. Dr R. Beyaert(1)(2). (1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium (2) VIB Center for Inflammation Research, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEXPR-myr-APEX (LMBP 9554) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. De Ceuleneer and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.