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GeneCorner plasmid details

Last data update: 22 July 2019 04:19 CEST  


Name: pdcDNA-R4R3-dhCMV   Add to cart
Accession number: LMBP 8998
Sequence file: LMBP sequence: p8998.gb, p8998.txt     View with Genome Compiler
Circular map: p8998.pdf
Status: GeneCorner core plasmid
Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Promoter: Escherichia coli lac operon promoter
Escherichia coli lac operon promoter; mutant (lacUV5)
Simian virus 40 early promoter (SV40 early)
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Bovine growth hormone polyadenylation signal (BGH polyA)
Selection marker: Ampicillin (amp)
Neomycin (neo; G418)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells; SV40 permissive cells
Parental clone: pcDNA3; pLenti6 pDEST pLenti + CMV MGW
Further information: This multisite Gateway destination vector was constructed by removing the human CMV-IE promoter from pcDNA3 as a BglII/XhoI fragment and replacing it with a multisite Gateway cassette containing attenuator sites attR4 and attR3.
The Gateway multisite cassette was PCR-amplified from pLenti6 pDEST pLenti + CMV MGW.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726807.1.
The region upstream of the ampicillin resistance gene and upstream of the f1 ori, containing the multisite Gateway cassette, has been sequenced by BCCM/GeneCorner.
EMBL Accession number: LT726807.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 03/02/2014
Authenticity test: Restriction enzyme pattern analysed by GeneCorner: EcoRI and HaeII.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. De Ceuleneer(1) and Prof. Dr R. Beyaert(1).
(1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12xB DB3.1
Host reference: -
Related host reference: Bernard et al., J. Mol. Biol. 226 (1992), 735-745 [PMID: 1324324]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)*
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin.
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

 

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