Last data update: 24 January 2024 16:39 CET
Plasmid name: pFANS1 (LMBP 2361)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pfans1.gb
(View with Genome Compiler) pfans1.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aspergillus nidulans ornithine carbamoyl transferase gene (argB) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Aspergillus nidulans ornithine carbamoyl transferase terminator (argB) |
| Selection marker: | Ampicillin (amp) Ornithine carbamoyl transferase (argB; auxotrophic) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Fungi; integrative |
| Parental clone: | pSal23; pDJB2 |
| Further information: | The plasmid was constructed as follows: The ans1 sequence was isolated as a 3.5 kb EcoRI fragment from pDJB2 and cloned into the unique EcoRI site of pSal43 (orientation not known). The 1.8 kb PvuII-PstI fragment of the ans1 sequence enhances the efficiency of transformation of this plasmid (4 to 10 times better as compared to pSal43). This plasmid also contains a 2.6 kb SalI fragment of Aspergillus nidulans DNA, containing the structural gene for ornithine carbamoyl transferase (argB). Some of the unique restriction sites mentioned are possibly not unique, because the nucleotide sequence is not completely known. Other name of the plasmid is pFANS. |
| EMBL Accession number: | - |
| Latest sequence update: | 21/10/1991 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII/NheI, EcoRI, HindIII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. Janssens(1) and Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Wouter Janssens (1989) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFANS1 (LMBP 2361) is available at BCCM/GeneCorner. This plasmid was deposited by W. Janssens and Prof. Dr R. Contreras and was published in Wouter Janssens, 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.