Last data update: 24 January 2024 16:39 CET
Plasmid name: pFGPDAngls2a (LMBP 5067)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5067.gb
(View with Genome Compiler) p5067.txt p5067.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aspergillus niger glucosidase ll gene (gls2); α subunit (gls2a) containing the first two introns |
| Promoter: | Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase promoter (gpdA) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Aspergillus nidulans tryptophan C terminator (trpC) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Filamentous fungi; integrative |
| Parental clone: | pFGPDTrgls2a; pUCAngls2a |
| Further information: | The plasmid was constructed by digesting plasmid pUCAngls2a with HindII and SpeI. The SpeI overhanging end was filled in using Klenow. A. niger gls2a was isolated and ligated into the SnaBI opened pFGPDTrgls2a vector, as such replacing the T. reesei gls2a open reading frame with that of A. niger (this sequence still contains the first two introns). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727078.1. The nucleotide sequence of the A. niger gls2a gene corresponds with the EMBL Nucleotide Sequence Database accession number AM270207.1. The nucleotide sequence of the pFGPD vector backbone corresponds with the EMBL Nucleotide Sequence Database accession number Z32699.1. Other name of the plasmid is pFGPDglsIIAniger. |
| EMBL Accession number: | AM270207.1, view at EMBL, GenBank, DDBJ Z32699.1, view at EMBL, GenBank, DDBJ LT727078.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 21/02/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, PvuI, PvuII and SacI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). It was constructed by S. Geysens(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFGPDAngls2a (LMBP 5067) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.