Last data update: 24 January 2024 16:39 CET
Plasmid name: pGAL1P:synthCaBAX (LMBP 4439)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4439.gb
(View with Genome Compiler) p4439.txt p4439.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); synthetic, Candida albicans codon adapted sequence (CaBAX) |
| Promoter: | Candida albicans galactokinase 1 promoter (CaGAL1) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans white colony-forming gene terminator (Wh11) |
| Selection marker: | Ampicillin (amp) Candida albicans URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Candida albicans; ura3(-), integrative |
| Parental clone: | pGAL1ACT1LUC |
| Further information: | The plasmid was constructed by inserting the CaBAX cDNA as a PstI/SmaI fragment between the PstI and StuI sites of pGAL1ACT1LUC. To facilitate the recognition of the AUG codon, a purine nucleotide (A) was introduced at position -3 (Kozak). pGAL1P:synthCaBAX is used for expression of CaBAX after linearisation with Bpu1102I (EspI) and integration in the Candida genome. As compared to the sequence of the pMB1 origin of pBR322, the sequence file of pGAL1P:synthCaBAX, which was obtained from the depositor, contains a ClaI site in the pMB1 region. This site has not been verified by BCCM/GeneCorner. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727209.1. The nucleotide sequence of the Candida Wh11 terminator corresponds with the EMBL Nucleotide Sequence Database accession number S65451.1. The nucleotide sequence of the CaURA3 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AF109400.1. This was confirmed by the sequence analysis results of BCCM/GeneCorner. Other names of the plasmid are pGAL1CaBAX and pGAL1P:synthCaBAX-(Kozak). |
| EMBL Accession number: | AF109400.1, view at EMBL, GenBank, DDBJ S65451.1, view at EMBL, GenBank, DDBJ LT727209.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 15/10/2013 |
| Sequence detail: | Nucleotide sequence at the start of the synthetic Candida BAX coding sequence:
CaGAL1 promoter->| |-> Candida BAX
5' ... AAAGAGTTATACC|CTGCAGGAAGATCATC|ATG.GAT.GGT.TCT. ... 3'
PstI ^ ***
^: nucleotide 'A' introduced at position -3 (Kozak).
***: start codon. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BamHI/PstI, EcoRI and HindIII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). It was constructed by I. Eberhardt(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Smet et al., Yeast 21 (2004), 1325-1334 [PMID: 15565645] |
| Related plasmid reference: | Kozak, Nucleic Acids Res. 9 (1981), 5233-5252 [PMID: 7301588] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGAL1P:synthCaBAX (LMBP 4439) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in De Smet et al., 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.