Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEX-4T-3-mTctex1 (LMBP 6816)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6816.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse t-complex testis-expressed 1 (Tctex1) Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST) Thrombin recognition site; N-terminal Escherichia coli lac repressor gene (lacI) Escherichia coli lac Z gene (lacZ); α part |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Escherichia coli lac repressor promoter; mutant (lacI(q)) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEX-4T-3 |
| Further information: | The plasmid was created by ligating the mTctex1 cDNA as an NcoI/NotI fragment into the NcoI/NotI opened pGEX-4T-3 vector. The nucleotide sequence of the mTctex1 cDNA corresponds with the Genbank accession number NM_009342.2. The nucleotide sequence of the pGEX-4T-3 vector corresponds with the EMBL Sequence Nucleotide Database accession number U13855.1. |
| EMBL Accession number: | U13855.1, view at EMBL, GenBank, DDBJ NM_009342.2, view at GenBank |
| Latest sequence update: | 21/01/2011 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BstZI, EcoRI, HaeII, HincII, NotI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by K. Staes(1) (2) and Prof. Dr F. Van Roy(1) (2). It was constructed by U. Fuchs(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEX-4T-3-mTctex1 (LMBP 6816) is available at BCCM/GeneCorner. This plasmid was deposited by K. Staes and Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.