Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEX-6P-3-hSC35(61-115)-S98A (LMBP 10243)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10243.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human serine and arginine rich splicing factor 2 cDNA (SRSF2, SFRS2, PR264, SC-35, SC35, SFRS2A, GeneID 6427); mutated fragment Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST); N-terminal Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Escherichia coli lac operon promoter Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEX-6P-3-hSC35(61-115) |
| Further information: | The plasmid was constructed by introducing an S98A mutation in the human SRSF2 coding sequence of pGEX-6P-3-hSC35(61-115). The plasmid is useful for chemically inducible, high-level expression of a GST-tagged fragment of human SRSF2 in bacterial cells. The GST domain can be separated from the human SRSF2 domain via cleavage with PreScission protease. The fragment of human SRSF2 consists of amino acids 61 up to and including 115 and contains an S98A mutation. The nucleotide sequence of the pGEX-6P-3 vector corresponds with the EMBL Nucleotide Sequence Database accession number U78874.1. Other name of the plasmid is pGEX-6P-3-hSC35(61-115), mut S98A. |
| EMBL Accession number: | U78874.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/08/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr S. Grootjans(1)(2) and Prof. Dr P. Vandenabeele(1)(2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEX-6P-3-hSC35(61-115)-S98A (LMBP 10243) is available at BCCM/GeneCorner. The plasmid was deposited by Dr S. Grootjans and Prof. Dr P. Vandenabeele. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.