Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEX-GST-ubiquitin Ile44Ala (LMBP 6072)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST) Human ubiquitin B cDNA (UBB, Ub, HEL-S-50, GeneID 7314); mutated mature sequence Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEX-GST-ubiquitin |
| Further information: | The plasmid contains a mutant ubiquitin cds where the isoleucin amino acid in position 44 was replaced by alanin. |
| EMBL Accession number: | BT020104, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/04/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr I. Dikic(1). (1) Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEX-GST-ubiquitin Ile44Ala (LMBP 6072) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Dikic. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.