Last data update: 24 January 2024 16:39 CET
Plasmid name: pIAHA (LMBP 1961)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | piaha.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Aichi/2/1968(H3N2)) haemagglutinin cDNA (HA, IVHA, IAHA); H3 subtype |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | cDNA of the A/Aichi/2/68 Influenza haemagglutinin gene (H3 subtype) was inserted in the unique PstI site of pBR322 by dA/dT-tailing. 27 nucleotides are missing at the end of the 3' untranslated region of IAHA. Name mentioned in Verhoeyen et al. (1980) is pXHA14. |
| EMBL Accession number: | - |
| Latest sequence update: | 07/12/1988 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Verhoeyen et al., Nature 286 (1980), 771-776 [PMID: 7402351] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIAHA (LMBP 1961) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Min Jou and was published in Verhoeyen et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.