Last data update: 24 January 2024 16:39 CET
Plasmid name: pIB1 (LMBP 5767)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5767.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Pichia pastoris HIS4 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris |
| Parental clone: | - |
| Further information: | pIB1 is a promoter-free integrating vector for expressing genes in Pichia pastoris. It contains a modified pMB1 replication origin and a modified pUC19 polylinker. The nucleotide sequence of the pIB1 vector corresponds with the EMBL Nucleotide Sequence Database accession number AF027958.1. |
| EMBL Accession number: | AF027958.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/03/2009 |
| Sequence detail: | Sequence of the modified pUC19 polylinker:
GAA.TTC.GAG.CTC.GGT.ACC.CGG.GGA.TCC.ACT.AGT.CTC.GAG.CTG.CAG.GCA.TGC.AAG.CTT
EcoRI BanII BanI SmaI BamHI SpeI XhoI PstI SphI HindIII
SacI KpnI XmaI |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr B. Glick(1). (1) Department of Molecular Genetics and Cell Biology, University of Chicago, USA |
| Plasmid reference: | Sears et al., Yeast 14 (1998), 783-790 [PMID: 9675822] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIB1 (LMBP 5767) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Glick and was published in Sears et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.