Last data update: 24 January 2024 16:39 CET
Plasmid name: pIB3 (LMBP 5769)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p5769.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Pichia pastoris YPT1 promoter |
Ribosome binding site: |
- |
Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Pichia pastoris HIS4 terminator |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Pichia pastoris |
Parental clone: | pIB1 |
Further information: | pIB3 is an integrating vector for constitutive gene expression in P. pastoris. It contains the moderately active constitutive promoter from the P. pastoris YPT1 gene. The plasmid contains a modified pUC19 polylinker. The nucleotide sequence of pIB3 was obtained from the EMBL Nucleotide Sequence Database accession number AF027960.1. |
EMBL Accession number: | AF027960.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 16/03/2009 |
Sequence detail: | Sequence of the modified pUC19 polylinker: GAA.TTC.GAG.CTC.GGT.ACC.CGG.GGA.TCC.ACT.AGT.CTC.GAG.CTG.CAG.GCA.TGC.AAG.CTT EcoRI BanII BanI SmaI BamHI SpeI XhoI PstI SphI HindIII SacI KpnI XmaI |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr B. Glick(1). (1) Department of Molecular Genetics and Cell Biology, University of Chicago, USA |
Plasmid reference: | Sears et al., Yeast 14 (1998), 783-790 [PMID: 9675822] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIB3 (LMBP 5769) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Glick and was published in Sears et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.