Last data update: 24 January 2024 16:39 CET
Plasmid name: pICOb (LMBP 9607)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p9607.gb
(View with Genome Compiler) p9607.txt p9607.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Escherichia coli β-lactamase promoter (amp) |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
Terminator: | - |
Selection marker: | Blasticidin (bsd) |
Replicon: | Escherichia coli plasmid pMB1 origin; truncated |
Host range: | Escherichia coli |
Parental clone: | pUCmu |
Further information: | The plasmid was constructed by PCR amplifying the pUCmu vector backbone without the coding sequence of the ampicillin resistance gene, and PCR amplifying the coding sequence of the blasticidin resistance gene. The two PCR fragments were subsequently fused with CloneEZ, effectively replacing the ampicillin resistance of pUCmu with blasticidin resistance. pICOb is a minimal cloning plasmid with an extended multiple cloning site. |
EMBL Accession number: | - |
Latest sequence update: | 28/02/2018 |
Sequence detail: | Sequence at the multiple cloning site: ...ACGCGTCGCGAGGCCATATGGGTTAACCCATGGCCAAGCTTGCATGCCTGC MluI NdeI HpaI NcoI HindIII PstI AGGTCGACTCTAGAGGATCCCGGGTACCGAGCTCGAATTCGGATATCCTCGAG SalI XbaI BamHI KpnI SacI EcoRI EcoRV XhoI XmaI RsaI ACTAGTGGGCCCGTTTAAAC... SpeI ApaI PmeI |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, PvuII/SpeI, PvuII/XhoI and RsaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. R. Beyaert(1) (2). (1) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + blasticidin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pICOb (LMBP 9607) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.