Last data update: 24 January 2024 16:39 CET
Plasmid name: pUCmu (LMBP 9329)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p9329.gb
(View with Genome Compiler) p9329.txt p9329.pdf |
| Sequence analysis results Genecorner: |
NGS: h11-gc-may2021.fasta |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin; truncated |
| Host range: | Escherichia coli |
| Parental clone: | pUCmini |
| Further information: | The plasmid was generated by multiple rounds of PCR and ligation, starting from pUCmini. First, the useless code between the ampicillin resistance gene and the pMB1 ori was deleted. Then, the restriction sites that were deleted during the generation of pUCmini were repaired. This PCR product was cut with XhoI prior to ligation to ensure correct repair. The resulting plasmid was test-digested in the repaired sites. This is a minimal cloning plasmid of only 1669 bp, while still having an extended multiple cloning site. Other name of the plasmid is pUCnano. |
| EMBL Accession number: | - |
| Latest sequence update: | 07/09/2020 |
| Sequence detail: | Primers used for the deletion of useless code between the ampicillin resistance gene and the pMB1 ori:
forward: 5' TACCAATGCTTAATCAGTGAGGCA 3'
reverse: 5' AGTAGAAAAGATCAAAGGATCTTCT 3'
Primers used to repair the restriction sites:
forward:
5' ATTAGCTCGAGACTAGTGGGCCCGTTTAAACACATGTGTTTTTCCATAGGTTCCG 3'
reverse:
5' CTAATCTCGAGGATATCCGAATTCGAGCTCGGTACCCGGGATCCTCTAGAGTCGACCTG 3'
Multi-cloning site of the pUCmu plasmid:
5' ACGCGTCGCGAGGCCATATGGGTTAACCCATGGCCAAGCTTGCATGCCTGCAGGTCGA
MluI NruI NdeI HpaI NcoI HindIII PstI SalI
MscI
CTCTAGAGGATCCCGGGTACCGAGCTCGAATTCGGATATCCTCGAGACTAGTGGGCCC
XbaI BamHI KpnI SacI EcoRI EcoRV XhoI SpeI ApaI
XmaI
GTTTAAACACATGTGT 3'
PmeI |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/PvuI/XhoI, DraI and HindIII. This plasmid has also been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Staal et al., BioTechniques 66 (2019), 254-259 [PMID: 31124712] [DOI: 10.2144/btn-2019-0014] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUCmu (LMBP 9329) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.