Last data update: 24 January 2024 16:39 CET
Plasmid name: pIDUHA (LMBP 2413)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
piduha.gb
(View with Genome Compiler) piduha.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A/duck/Ukraine/63 haemagglutinin cDNA (IDUHA); fragment |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | cDNA of the A/duck/Ukraine 63 influenza haemagglutinin gene (IDUHA) was inserted in the unique PstI of pBR322 by dA-dT tailing. 196 nucleotides are missing at the 3' end of the gene, including the termination codon and the last 53 amino acid codons. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727513.1. Name mentioned in Fang et al. (1981) is pDHA18. |
| EMBL Accession number: | LT727513.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/03/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AccI, BglII and EcoRI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Fang et al., Cell 25 (1981), 315-323 [PMID: 6169439] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIDUHA (LMBP 2413) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Min Jou and was published in Fang et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.