Last data update: 24 January 2024 16:39 CET
Plasmid name: pIVHA (LMBP 1960)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pivha.gb
(View with Genome Compiler) pivha.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) haemagglutinin cDNA (HA, IVHA) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | cDNA of the A/Victoria/3/75 influenza haemagglutinin gene (IVHA) was inserted in the unique PstI site of pBR322 by dA/dT-tailing. 24 nucleotides at the end of the 3' UTR of IVHA are missing. This wild type haemagglutinin coding sequence is also called membrane-bound haemagglutinin (HAm); it includes the sequences coding for the intramembranous part and the cytoplasmic tail of the protein. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727608.1. Name mentioned in Min Jou et al. (1980) is pVHA14. |
| EMBL Accession number: | LT727608.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/12/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/XmnI, EcoRV/XbaI, HindII/SpeI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Min Jou et al., Cell 19 (1980), 683-696 [PMID: 6153930] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIVHA (LMBP 1960) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Min Jou and was published in Min Jou et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.