Last data update: 24 January 2024 16:39 CET
Plasmid name: pJE202 (LMBP 4113)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4113.gb
(View with Genome Compiler) p4113.txt p4113.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Vibrio fischeri luxR gene Vibrio fischeri luxICDABEG operon consisting of the luxI, luxC, luxD, luxA, luxB, luxE and luxG genes |
| Promoter: | Vibrio fischeri luxR promoter Vibrio fischeri luxICDABEG operon promoter |
| Ribosome binding site: |
Ribosome binding sites (RBS) of the Vibrio fischeri luxR, luxI, luxC, luxD, luxA, luxB, luxE and luxG genes |
| Terminator: | Vibrio fischeri luxICDABEG operon terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBR322 |
| Further information: | This plasmid was constructed by cloning a 9kb SalI fragment of V. fischeri MJ-1, containing the luxR gene and the luxICDABEG operon consisting of the luxI, luxC, luxD, luxA, luxB, luxE and luxG genes, into the SalI opened pBR322. The luxR gene and the luxICDABEG operon are transcribed divergently, respectively from the luxR and the luxICDABEG operon promoter that are located in between the luxR and the luxI genes. The lux promoters and RBS sites are not shown on the circular map of the plasmid. pJE202 contains all functions required for spontaneous light emission; addition of external substrate is not necessary. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727306.1. The nucleotide sequence of the inserted SalI fragment corresponds with the EMBL Nucleotide Sequence Database accession number AF170104.1. |
| EMBL Accession number: | AF170104.1, view at EMBL, GenBank, DDBJ LT727306.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/04/2000 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, EcoRI/XbaI and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Engebrecht(1). It was constructed by Dr J. Engebrecht and Dr M. Silverman(2) in 1982. (1) Department of Pharmacological Sciences, State University of New York, Suny-Stonybrook, New York, USA (2) Agouron Institute, La Jolla, California, USA |
| Plasmid reference: | Engebrecht et al., Cell 32 (1983), 773-781 [PMID: 6831560] |
| Related plasmid reference: | Engebrecht et al., Nucleic Acids Res. 15 (1987), 10455-10467 [PMID: 3697093] Engebrecht et al., Proc. Natl. Acad. Sci. U.S.A. 81 (1984), 4154-4158 [PMID: 6377310] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli YMC10 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (80 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | Cultivate for at least 20 hours. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pJE202 (LMBP 4113) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Engebrecht and was published in Engebrecht et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.