Last data update: 24 January 2024 16:39 CET
Plasmid name: pJH2 (LMBP 10348)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10348.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP), C-terminal |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Broad-host-range Gram-negative Bordetella bronchiseptica S87 plasmid pBBR1 replicon |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Parental clone: | pAP20 |
| Further information: | The plasmid was constructed by cloning the EGFP reporter coding sequence into the pAP20 vector. The plasmid is intended for the construction of translational fusions with the EGFP reporter in Gram-negative bacteria. The plasmid does not include a promoter, and the EGFP coding sequence lacks a starting codon. The nucleotide sequence of the pJH2 vector corresponds with the EMBL Nucleotide Sequence Database accession number FJ607244.1. |
| EMBL Accession number: | FJ607244.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 08/05/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr A. Sass(1). It was obtained from Prof. Dr S. Cardona(2). (1) Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium (2) Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada |
| Plasmid reference: | Hamlin et al., BMC Microbiol. 9 (2009), 222 [PMID: 19835630] [DOI: 10.1186/1471-2180-9-222] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pJH2 (LMBP 10348) is available at BCCM/GeneCorner. The plasmid was deposited by Dr A. Sass and was published in Hamlin et al., 2009. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.