Last data update: 24 January 2024 16:39 CET
Plasmid name: pLM21 (LMBP 9305)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9305.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Yersinia enterocolitica secretion protein P gene (yscP); modified sequence (yscP686) Escherichia coli lac repressor gene (lacI) Histidine tag (His-tag); N-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) Escherichia coli lac repressor promoter (lacI) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Streptomycin (Sm; spectinomycin (Sp)) |
| Replicon: | Enterobacter cloacae plasmid ClDF13 origin |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Parental clone: | pCDFDuet; pSTW14 |
| Further information: | The plasmid was constructed by PCR amplifying the Y. enterocolitica yscP686 CDS from pSTW14 and cloning it into the NdeI/EcoRI opened pCDFDuet vector. Y. enterocolitica yscP686 is a derivative of yscP in which an additional copy of codons 214-374 has been inserted between codons 250 and 251. Other name of the plasmid is pDUET: yscp686+. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/07/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr L. Metzger(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Biozentrum, University of Basel, Switzerland |
| Plasmid reference: | Wagner et al., Proc. Natl. Acad. Sci. U.S.A. 107 (2010), 13860-13865 [PMID: 20643949] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 LK111 |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + streptomycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLM21 (LMBP 9305) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Wagner et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.