Last data update: 24 January 2024 16:39 CET
Plasmid name: pLPV3Z (LMBP 12691)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p12691.gb
(View with Genome Compiler) p12691.txt p12691.pdf |
| Sequence analysis results Genecorner: |
NGS: e11-gc-may2021.fasta |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); mutated sequence Acinetobacter calcoaceticus putative toxin cDNA (parE2-like) Acinetobacter calcoaceticus putative antitoxin cDNA (paaA2-like) |
| Promoter: | Escherichia coli β-lactamase promoter (amp) Escherichia coli plasmid R388 class 1 integron Pc promoter (PcS) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) Ribosome binding site (RBS); synthetic sequence |
| Terminator: | Phage λ Rho-independent termination signal |
| Selection marker: | Gentamicin (Gm) Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Acinetobacter calcoaceticus plasmid pWH1277 origin |
| Host range: | Escherichia coli Acinetobacter spp. |
| Parental clone: | pVRL1 |
| Further information: | The construction of the plasmid is described in Lucidi et al. (2019). This plasmid is a promoter-probe E. coli-Acinetobacter species shuttle vector. Promoter activity in Acinetobacter can be evaluated based on a fast-turnover variant of GFP. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MK681508.1, except for a missing nucleotide upstream of of the amp promoter, and 4 additional nucleotides downstream of the zeocin resistance gene, corresponding the NGS sequence analysis results. |
| EMBL Accession number: | MK681508.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/01/2022 |
| Authenticity test: | This plasmid has been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. M. Lucidi (1) and Prof. P. Visca (1). (1) Faculty of Science, University of Roma Tre, Rome, Italy |
| Plasmid reference: | Lucidi et al., Appl. Environ. Microbiol. 85 (2019), e01334-19 [PMID: 31324623] [DOI: 10.1128/AEM.01334-19] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + gentamycin (12.5 μg/ml) + zeocin (25μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLPV3Z (LMBP 12691) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. M. Lucidi and Prof. P. Visca and was published in Lucidi et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.