Last data update: 24 January 2024 16:39 CET
Plasmid name: pLT32hC14wt (LMBP 5337)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5337.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human apoptosis-related cysteine peptidase 14 cDNA (CASP14, caspase 14, caspase-14, CASP-14) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLT32 |
| Further information: | The plasmid was constructed by cloning the human CASP14 CDS into the pLT32 vector. This low copy number plasmid can be used for IPTG-inducible expression of human CASP14 after transformation into an E. coli strain containing a compatible piCA plasmid. Expression of human CASP14 with this vector can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly. The nucleotide sequence of the human CASP14 CDS corresponds with the EMBL Nucleotide Sequence Database accession number EU502748.1. |
| EMBL Accession number: | EU502748.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/10/2006 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Hoste et al., J. Invest. Dermatol. 131 (2011), 2233-2241 [PMID: 21654840] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
| Related website: | http://www.irc.ugent.be/index.php?id=proteinservicehome |
Refer in your Materials and Methods: |
pLT32hC14wt (LMBP 5337) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. De Groote and Prof. Dr W. Declercq and was published in Hoste et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.