Last data update: 24 January 2024 16:39 CET
Plasmid name: pMC1403 (LMBP 474)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pmc1403.gb
(View with Genome Compiler) pmc1403.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); not functional Escherichia coli lac Y gene (lacY) Escherichia coli lac A gene (lacA) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use lacZ(-) strains |
| Parental clone: | pBR322 |
| Further information: | The plasmid can be used for cloning promoters with translation-initiation sequences and analyse their expression level by means of synthesis of an active β-galactosidase fusion protein. The lacZ gene is not complete (the first 8 amino acids are missing). The EcoRI site in the lacZ gene was removed. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727182.1. Other name of the plasmid is pMC936. |
| EMBL Accession number: | LT727182.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/03/1992 |
| Sequence detail: | Nucleotide sequence in front of the lacZ gene:
9
5' GA.ATT.CCC.GGG.GAT.CCC.GTC.GTT 3'
EcoRI SmaI BamHI *
*: Ninth codon of the lacZ gene.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M.J. Casadaban(1). (1) University of Chicago, USA |
| Plasmid reference: | Casadaban et al., J. Bacteriol. 143 (1980), 971-980 [PMID: 6162838] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M182 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | NCCB 3088 PC-V 3088 |
Refer in your Materials and Methods: |
pMC1403 (LMBP 474) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M.J. Casadaban and was published in Casadaban et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.