Last data update: 24 January 2024 16:39 CET
Plasmid name: pMH30 (LMBP 7957)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p7957.gb
(View with Genome Compiler) p7957.txt p7957.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Photorhabdus luminescens luciferase alpha subunit DNA (luxA) Photorhabdus luminescens luciferase beta subunit DNA (luxB) Photorhabdus luminescens fatty acid reductase DNA (luxC) Photorhabdus luminescens acyl transferase DNA (luxD) Photorhabdus luminescens acyl-protein synthetase DNA (luxE) |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid R6K origin; defective pir gene |
| Host range: | Escherichia coli; use strains supplying the R6K pir function |
| Parental clone: | pCS26-Pac; pCD13PKS |
| Further information: | This light-producing reporter plasmid was constructed by inserting a PacI PCR amplicon from pCD13PKS, containing the E. coli R6K pir(-) ori and the phage λ attP3 site, into the PacI opened pCS26-Pac vector. The nucleotide sequence of the pMH30 vector corresponds with the EMBL Nucleotide Sequence Database accession number JF420885.1. |
| EMBL Accession number: | JF420885.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/11/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/SacI, HaeII, HindIII and SacII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr M. Herrington(1). (1) Biology Department, Concordia University, Montreal, Canada |
| Plasmid reference: | Sitaras et al., Plasmid 65 (2011), 232-238 [PMID: 21376749] [DOI: 10.1016/j.plasmid.2011.02.005] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αpir116 variant |
| Host reference: | Platt et al., Plasmid 43 (2000), 12-23 [PMID: 10610816] |
| Related host reference: | Sitaras et al., Plasmid 65 (2011), 232-238 [PMID: 21376749] [DOI: 10.1016/j.plasmid.2011.02.005] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Miller + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMH30 (LMBP 7957) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr M. Herrington and was published in Sitaras et al., 2011. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.