Last data update: 24 January 2024 16:39 CET
Plasmid name: pMSCV-F-BirA (LMBP 5689)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5689.gb
(View with Genome Compiler) p5689.txt p5689.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli biotin protein ligase cDNA (birA) FLAG epitope tag; N-terminal |
| Promoter: | Mouse stem cell PCMV virus long terminal repeat (5' MSCV-PCMV LTR) Mouse phosphoglycerate kinase 1 promoter (PGK1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Mouse stem cell PCMV virus long terminal repeat (3' MSCV-PCMV LTR) |
| Selection marker: | Ampicillin (amp) Puromycin (puro) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells |
| Parental clone: | pMSCV-FLAG-puromycin |
| Further information: | The plasmid contains the mouse stem cell PCMV (PCC4-cell-passaged myeloproliferative sarcoma virus) virus long terminal repeat. The LTR drives high-level constitutive expression of BirA in stem cells and other mammalian cell lines. The plasmid contains also the extended retroviral packaging signal, Ψ+, which promotes high-titer production of replication-incompetent infectious viral particles after introduction into a packaging cell line. The plasmid is used in cotransfection with clones carrying the bio-tag for purification of biotinylated proteins. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726944.1. Other names of the plasmid are MSCV-BirA and MSCVp-F-BirA. |
| EMBL Accession number: | LT726944.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/11/2007 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AatII/KpnI, Alw44I/XbaI, Bst98I, NcoI, PvuII/SpeI, SphI, SspI and Tth111I. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M. Baens(1). (1) Department of Human Genetics, KULeuven, Belgium |
| Plasmid reference: | Noels et al., J. Biol. Chem. 282 (2007), 10180-10189 [PMID: 17287209] |
| Related plasmid reference: | Alrefai et al., Nat. Commun. 7 (2016), 11724 [PMID: 27222343] [DOI: 10.1038/ncomms11724] Muhammad et al., Front. Immunol. 9 (2018), 32 [PMID: 29416540] [DOI: 10.3389/fimmu.2018.00032] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Cultivation remark: | Take a low-copy-plasmid yield into account while choosing a plasmid DNA isolation procedure. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMSCV-F-BirA (LMBP 5689) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M. Baens and was published in Noels et al., 2007. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.