Last data update: 24 January 2024 16:39 CET
Plasmid name: pMxh5HT2R (LMBP 3029)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3029.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human serotonin 5-HT2 receptor (h5HT2R) |
| Promoter: | Mouse Mx promoter Escherichia coli lac operon promoter Phage SP6 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pSP64MxpA; UCh5HT2R |
| Further information: | The plasmid was constructed as follows : pSP64MxpA was digested with XbaI / BglI and with BamHI / BglI to isolate the Mx-promoter and the SV40 intron and the polyA-sequences respectively. The human serotonin 5HT2 receptor was isolated as a NheI/BamHI fragment from pUCh5HT2R. Ligation of the three fragments resulted in pMxh5HT2R. This plasmid allows functional expression of the human serotonin 5HT2 receptor in L929s(A). The Mx promoter is inducible with IFNα and IFNβ. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other name of the plasmid is pMx5HT2. |
| EMBL Accession number: | - |
| Latest sequence update: | 10/08/1994 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed in the research unit of Em. Prof. Dr G. Haegeman(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMxh5HT2R (LMBP 3029) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.