Last data update: 24 January 2024 16:39 CET
Plasmid name: pPAS6 (LMBP 2134)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | ppas6.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Escherichia coli papA gene (papA); mutant 68B |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene; hybrid with papA SD |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc245; pPAS3 |
Further information: | This plasmid was constructed as follows : The EcoRI-XbaI fragment of pPAS3 (containing the 68B mutant of E. coli papA gene) was inserted between the EcoRI and XbaI sites of pPLc245. Other name of the plasmid is pPLc245PAPA68B. |
EMBL Accession number: | - |
Latest sequence update: | 09/06/1993 |
Sequence detail: | Nucleotide sequence of the mutated papA gene: 60 68 69 70 GTT AAT TGT GAT ATT ACT GCC TTT AAA GGG GAT CCT GGC GCC AAA AAA Val Asn Cys Asp Ile Thr Ala Phe Lys Gly Asp Pro Gly Ala Lys Lys BamHI E. coli papA gene wt : 60 68 69 70 GTT AAT TGT GAT ATT ACT GCC TTT AAA GGT GGT AAT GGC GCC AAA AAA Val Asn Cys Asp Ile Thr Ala Phe Lys Gly Gly Asn Gly Ala Lys Lys |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Steidler et al., J. Bacteriol. 175 (1993), 7639-7643 [PMID: 7902348] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061(λ) |
Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPAS6 (LMBP 2134) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut and was published in Steidler et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.