Last data update: 24 January 2024 16:39 CET
Plasmid name: pPAS8 (LMBP 2131)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | ppas8.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mutant 68B of Escherichia coli papA gene (papAm) - A domain of Staphylococcus aureus spa gene fusion |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene; hybrid with papA SD |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPAS6; pMcSPA2 |
| Further information: | This plasmid was constructed as follows : the small BamHI fragment of pMcSPA2 (containing the A domain of the S. aureus spa gene) was inserted into the unique BamHI site, created at AA 68 of the papaA gene, of pPAS6. Other name of the plasmid is pPLc245PAPA68BSPAA. |
| EMBL Accession number: | - |
| Latest sequence update: | 10/06/1993 |
| Sequence detail: | Nucleotide sequence around the gene fusion:
papA ->| |<- spa
| |
64 65 66 67 68 linker 111 112
ACT GCC TTT AAA GGG GAT CCC AAT TTC AAC AAA GAA CAA CAA AAT GCT
Thr Ala Phe Lys Gly Asp Pro Asn Phe Asn Lys Glu Gln Gln Asn Ala
BamHI
120 130
TTC TAT GAA ATC TTG AAT ATG CCT AAC TTA AAC GAA GAA CAA CGC AAT
Phe Tyr Glu Ile Leu Asn MET Pro Asn Leu Asn Glu Glu Gln Arg Asn
140 150
GGT TTC ATC CAA AGC TTA AAA GAT GAC CCA AGC CAA AGT GCT AAC CTA
Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu
spa ->|
|
A ->|<- B
160 165|166 167
TTG TCA GAA GCT AAA AAG TTA AAT GAA TCT CAA GCA CCG AAA GCG GAT
Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala Asp
BamHI
|<- papA
|
linker 71 72 73 74
CCT GGC GCC AAA AAA
Pro Gly Ala Lys Lys
Nucleotide sequence of the (mutated) A domain of S. aureus
spa gene in pMcSPA2 :
D ->|<- A
| 110
CAA GCA CCG AAA GCG GAT CCC AAT TTC AAC AAA GAA CAA CAA AAT GCT
Gln Ala Pro Lys Ala Asp Pro Asn Phe Asn Lys Glu Gln Gln Asn Ala
BamHI
120 130
TTC TAT GAA ATC TTG AAT ATG CCT AAC TTA AAC GAA GAA CAA CGC AAT
Phe Tyr Glu Ile Leu Asn MET Pro Asn Leu Asn Glu Glu Gln Arg Asn
140 150
GGT TTC ATC CAA AGC TTA AAA GAT GAC CCA AGC CAA AGT GCT AAC CTA
Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu
A ->|<- B
160 |
TTG TCA GAA GCT AAA AAG TTA AAT GAA TCT CAA GCA CCG AAA GCG GAT
Leu Ser Glu Ala Lys Lys Leu Asn Glu Ser Gln Ala Pro Lys Ala Asp
BamHI
170
CCC AAA TTC AAC AAA GAA
Asn Lys Phe Asn Lys Glu |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Steidler et al., J. Bacteriol. 175 (1993), 7639-7643 [PMID: 7902348] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061[pcI857] |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Helper plasmid: | pcI857 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | LMBP 02129 |
Refer in your Materials and Methods: |
pPAS8 (LMBP 2131) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut and was published in Steidler et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.