Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLa831 (LMBP 340)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p340.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLa83 |
| Further information: | As compared to pPLa2311, this plasmid provides no resistance to ampicillin. Expression sites: ApaLI, BamHI, EcoRI and MstI, which are all unique. The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. Other name of the plasmid is pHS31. |
| EMBL Accession number: | - |
| Latest sequence update: | 24/08/1986 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AluI, Alw44I, HaeII/HindIII and HincII/NaeI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M5219 |
| Host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLa831 (LMBP 340) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.