Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc245HIFNB25 (LMBP 672)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p672.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β cDNA (IFNB, IFNB1); mature sequence |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc245; pPLcRX1919 |
| Further information: | pPLc245HIFNB25 is an expression vector for human IFNB. The plasmid contains contains the mature human interferon β sequence, preceeded in a functional way by the ribosome binding site of the phage MS2 polymerase gene and the phage λ PL promoter . The nucleotide sequence of the human IFNβ DNA corresponds with the EMBL Nucleotide Sequence Database accession number V00546.1. Other name of the plasmid is pPLc245-HFIF25. |
| EMBL Accession number: | V00546.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 11/03/1987 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555] PhD thesis Patrick Stanssens (1985) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 ΔH1Δtrp |
| Host reference: | Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049] |
| Related host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc245HIFNB25 (LMBP 672) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Patrick Stanssens, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.