Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc28LIG8 (LMBP 574)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p574.gb
(View with Genome Compiler) p574.txt p574.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage T4 ligase gene |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T4 ligase gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc28; λNM540T4lig |
| Further information: | The plasmid contains the T4 ligase enzyme coding region, cloned as a HindIII fragment from phage λNM540T4lig (Wilson et al. (1979)) in the HindIII site of pPLc28, in the sense orientation relative to the λ PL promoter. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726946.1. The nucleotide sequence of the phage T4 ligase gene corresponds with the EMBL Nucleotide Sequence Database accession number X00039.1. Name mentioned in Remaut et al. (1983) is pPLc28lig8. Other name of the plasmid is pST28lig8. |
| EMBL Accession number: | X00039.1, view at EMBL, GenBank, DDBJ LT726946.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/03/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, HindIII and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., Gene 22 (1983), 103-113 [PMID: 6305768] |
| Related plasmid reference: | Wilson et al., J. Mol. Biol. 132 (1979), 471-491 [PMID: 160464] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 C600[pcI857] |
| Host reference: | - |
| Related host reference: | Appleyard, Genetics 39 (1954), 440-452 [PMID: 17247495] |
| Helper plasmid: | pcI857 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc28LIG8 (LMBP 574) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.