Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc35 (LMBP 723)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pplc35.gb
(View with Genome Compiler) pplc35.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc32; pPLc2819 |
| Further information: | This plasmid has two XbaI expression sites in the polylinker downstream from the λ PL promoter. E-PstI, E-HindIII, E-XbaI sites are not unique. The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. |
| EMBL Accession number: | - |
| Latest sequence update: | 13/08/1986 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindII, PvuI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Eugeen Vanmechelen (1986) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc35 (LMBP 723) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Eugeen Vanmechelen, 1986. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.