Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLcmuMHTNF4 (LMBP 2530)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2530.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse tumor necrosis factor (Tnf, TNF); fragment Human tumor necrosis factor (TNF); fragment |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage Mu ner protein gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLcmuHTNF1; pATTrpMTNF |
| Further information: | This plasmid was constructed as follows : the NcoI-BanI fragment of pATTrpMTNF (containing the first part of mature mouse TNF) was inserted into the NcoI-BanI sites of pPLcmuHTNF1. pPLcmuMHTNF4 is designed for a PL-controlled expression of intracellular mature human-mouse hybrid TNF. Restriction analysis revealed that the plasmid is approx. 1600 bp shorter as described. Supposedly the plasmid is derived from a pPLc245HTNF-plasmid and not from pPLcmuHTNF1. The nucleotide sequence file has not been adapted. Other names of the plasmid are MH4 and pPLc245-MH4. |
| EMBL Accession number: | M11731, view at EMBL, GenBank, DDBJ X01394, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/01/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tavernier et al., J. Mol. Biol. 211 (1990), 493-501 [PMID: 1689779] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH1(λ) |
| Host reference: | Bachmann, in 'Escherichia coli and Salmonella: Cellular and Molecular Biology', American Society for Microbiology, Washington DC (1987), 1190-1219 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLcmuMHTNF4 (LMBP 2530) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Tavernier et al., 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.