Last data update: 24 January 2024 16:39 CET
Plasmid name: pSC2D (LMBP 2892)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psc2d.gb
(View with Genome Compiler) psc2d.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Saccharomyces cerevisiae iso-1-cytochrome C promoter (CYC1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic Methotrexate (MTX) |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae autonomously replicating sequence (ARS1) Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pEX2; pSV2mDHFR |
| Further information: | The resistance level to methotrexate (MTX) is low. Expression sites: BamHI, EcoRI and SmaI. Only the BamHI site is unique. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727554.1. Other name of the plasmid is pEXd3. |
| EMBL Accession number: | LT727554.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/05/1990 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRV and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Zhu(1) and Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Zhu et al., Biotechnology 3 (1985), 451-456 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSC2D (LMBP 2892) is available at BCCM/GeneCorner. This plasmid was deposited by J. Zhu and Prof. Dr R. Contreras and was published in Zhu et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.