Last data update: 24 January 2024 16:39 CET
Plasmid name: pEX2 (LMBP 2890)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2890.gb
(View with Genome Compiler) p2890.txt p2890.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Saccharomyces cerevisiae iso-1-cytochrome C promoter (CYC1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Saccharomyces cerevisiae autonomously replicating sequence (ARS1) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pEX1 |
| Further information: | This is an expression vector for yeast. Expression sites: BamHI, EcoRI and SmaI. Only the BamHI site is unique. This plasmid is different from pEX2, accession numbers NCCB 3076 and PC-V3076. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727553.1. Name mentioned in Ernst et al. (1985) is pEX-2. |
| EMBL Accession number: | LT727553.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/12/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, EcoRV, NcoI/XmnI, NdeI, SalI, SphI and XbaI. As compared to the fragments deduced from the compiled nucleotide sequence, the 334 bp XmnI/NcoI fragment of the 5' CYC1 promoter region seems to be rather approximately 200 bp long. Referring to other plasmids containing the CYC1 promoter region without this XmnI/NcoI fragment, we assume that the CYC1 promoter in pEX2 is functional (not experimentally verified). |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Ernst(1). (1) Institut für Mikrobiologie, Heinrich-Heine Universit“t, Düsseldorf, Germany |
| Plasmid reference: | Ernst et al., J. Bacteriol. 163 (1985), 8-14 [PMID: 2989254] |
| Related plasmid reference: | Chen et al., Exp. Gerontol. 38 (2003), 1051-1063 [PMID: 14580858] [DOI: 10.1016/s0531-5565(03)00186-4] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEX2 (LMBP 2890) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Ernst and was published in Ernst et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.