Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCGALyEGFP2 (LMBP 4205)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4205.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); yeast enhanced sequence (yEGFP) |
| Promoter: | Saccharomyces cerevisiae galactokinase promoter (GAL1) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) HIS3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; his3(-), integrative |
| Parental clone: | pSCGALyEGFP1; YDp-H |
| Further information: | The plasmid was constructed as follows: the BamHI fragment (blunted with Klenow DNA polymerase) of YDp-H, containing the HIS3 gene, was ligated to the MluI-Ecl136II fragment (blunted with T4 DNA polymerase) of pSCGALyEGFP1. pSCGALyEGFP2 is an integrative reporter plasmid for yEGFP in S. cerevisiae. After KpnI linearisation in its HIS3 element, single-copy genomical integration leads to stable expression. Other name of the plasmid is YIpUTH-GFP. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/03/2003 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCGALyEGFP2 (LMBP 4205) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.