Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCMF3 (LMBP 2893)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pscmf3.gb
(View with Genome Compiler) pscmf3.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) |
| Promoter: | Saccharomyces cerevisiae α-mating factor 1 promoter (MF) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUC8 |
| Further information: | The unique StuI site at the end of the prepro sequence was introduced by mutation . The segment from residue 149 until 401 corresponds in anti- clockwise orientation to the 3' end of the lacZ alpha fragment. ATTENTION : the vector part of the plasmid is probably not correct: the restriction sites were not experimentally verified. Usable fragment: EcoRI-StuI fragment or BglII-StuI fragment. Only the first spacer sequence (to HindIII) is on the plasmid. Because of the presence of the lac operator, lac+ E. coli host cells turn blue on X-Gal. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. . The nucleotide sequence from nucleotide position 1620 to 1320 corresponds with the upstream sequence of the yeast α-mating factor 1 (MF) promoter. Other name of the plasmid is pMATA2151H2. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/11/1989 |
| Sequence detail: | Nucleotide sequence at the end of the prepro sequence:
---- ppMF ---->|
80 83| |
5' GTA.TCT.TTG.GAT.AAA.AGG|CCT.CTT.GAA.GCT.TGG 3'
Val Ser Leu Asp Lys Arg|Pro Ala Glu Ala Trp
*|*** **
StuI HindIII
*: mutated nucleotide
|: Cutting site of the alpha mating factor KEX2 endoprotease. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, BglII, EcoRI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Kurjan et al., Cell 30 (1982), 933-943 [PMID: 6754095] Hynes et al., Mol. Cell. Biol. 3 (1983), 1430-1439 [PMID: 6353203] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCMF3 (LMBP 2893) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.