Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC8 (LMBP 1357)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
puc8.gb
(View with Genome Compiler) puc8.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); α part |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening |
| Parental clone: | pBR322 |
| Further information: | pUC8 is similar to pUC18, but contains fewer cloning sites in the lacZα region. Designed as an insert detection vector using α-complementation recently used for the expression of fused proteins: ATG in phase with GTC from E-AccI, E-HindII, E-SalI. ATG in phase with CGG from E-AvaI and E-SmaI. ATG in phase with GGA from E-BamHI. ATG in phase with CTG from E-BspMI. ATG in phase with AAT from E-EcoRI. ATG in phase with AGC from E-HindIII. ATG in phase with CTG from E-PstI. pUC8 has no PstI site in the ampicillin resistance gene. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database entry (Release 37; Accession number L08959), starting at another nucleotide position and complemented. |
| EMBL Accession number: | L08959, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/02/1989 |
| Sequence detail: | Nucleotide sequence around the polylinker:
5' ATG.ACC.ATG.ATT.ACG.AAT.TCC.CGG.GGA.TCC.GTC.GAC.CTG.CAG.CGA.AGC.TTG 3'
* EcoRI SmaI BamHI SalI PstI HindIII
AvaI HindII
AccI
BspMI
*: Start codon of lacZalpha.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, PvuI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Messing(1). (1) State University of New Jersey, Piscataway, USA |
| Plasmid reference: | Messing, Gene 19 (1982), 269-276 [PMID: 6295880] |
| Related plasmid reference: | Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | DSM 3420 NCCB 3165 PC-V 3165 |
Refer in your Materials and Methods: |
pUC8 (LMBP 1357) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Messing and was published in Messing, 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.