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GeneCorner plasmid details

Last data update: 03 December 2021 13:45 CET

Plasmid name: pUC8 (LMBP 1357)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: puc8.gb (View with Genome Compiler)
puc8.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: Escherichia coli lac Z gene (lacZ); α part
Promoter: Escherichia coli lac operon promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli; use strains expressing the lacZ ω fragment (lacZΔM15) for screening
Parental clone: pBR322
Further information: pUC8 is similar to pUC18, but contains fewer cloning sites in the lacZα region.
Designed as an insert detection vector using α-complementation recently used for the expression of fused proteins:
ATG in phase with GTC from E-AccI, E-HindII, E-SalI.
ATG in phase with CGG from E-AvaI and E-SmaI.
ATG in phase with GGA from E-BamHI.
ATG in phase with CTG from E-BspMI.
ATG in phase with AAT from E-EcoRI.
ATG in phase with AGC from E-HindIII.
ATG in phase with CTG from E-PstI.
pUC8 has no PstI site in the ampicillin resistance gene.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database entry (Release 37; Accession number L08959), starting at another nucleotide position and complemented.
EMBL Accession number: L08959, view at EMBL, GenBank, DDBJ
Latest sequence update: 01/02/1989
Sequence detail:
Nucleotide sequence around the polylinker:

5' ATG.ACC.ATG.ATT.ACG.AAT.TCC.CGG.GGA.TCC.GTC.GAC.CTG.CAG.CGA.AGC.TTG 3'
   *                 EcoRI  SmaI   BamHI   SalI    PstI      HindIII
                            AvaI           HindII
                                           AccI
                                                BspMI

*: Start codon of lacZalpha.
Punctuation indicates reading frame.
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BamHI, PvuI and TaqI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr J. Messing(1).
(1) State University of New Jersey, Piscataway, USA
Plasmid reference: Messing, Gene 19 (1982), 269-276 [PMID: 6295880]
Related plasmid reference: Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 RR1
Host reference: -
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: DSM 3420
NCCB 3165
PC-V 3165

Refer in your Materials and Methods:

pUC8 (LMBP 1357) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Messing and was published in Messing, 1982.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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