Last data update: 24 January 2024 16:39 CET
Plasmid name: pSP65HIL4 (LMBP 1919)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1919.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 4 cDNA (IL4, IL-4, BCGF-1, BCGF1, BSF1, GeneID 3565) |
| Promoter: | Phage SP6 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP65HIL4f |
| Further information: | This plasmid was derived from pSP65HIL4f2 (the same plasmid as pSP65HIL4f but with hIL4f in the clockwise orientation) as follows: The EcoRI site near the SP6 promoter was converted to an Asp700 (XmnI) site by partial digestion and filling in with Klenow DNA polymerase, resulting in pSP65HIL4f3 having a single EcoRI site present in the coding sequence of mature hIL4. In this EcoRI site the second EcoRI fragment of the EcoRI linked hIL4 cDNA (from the same λgt10 phage) was inserted (the second EcoRI linker was placed approx. 90 nucleotides behind the termination codon of the hIL4 gene). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of the hIL4 cDNA was assumed to be identical to the sequence reported by Yokota et al. (1986). |
| EMBL Accession number: | - |
| Latest sequence update: | 13/02/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Jayaram et al., Gene 79 (1989), 345-354 [PMID: 2676727] |
| Related plasmid reference: | Yokota et al., Proc. Natl. Acad. Sci. U.S.A. 83 (1986), 5894-5898 [PMID: 3016727] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSP65HIL4 (LMBP 1919) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Jayaram et al., 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.