Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV-Sport-di-RF (LMBP 5253)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5253.gb
(View with Genome Compiler) p5253.txt |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Renilla reniformis luciferase gene (rLUC) Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) |
| Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter; mutant (lacUV5) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV-Sport-Rluc; pSV-Sport-Fluc |
| Further information: | When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726984.1. To create the empty dicistronic vector pSV-Sport-di-RF, the firefly luciferase coding region was excised as a SmaI/XbaI fragment from pSV-Sport-Fluc and inserted downstream from the Renilla luciferase coding region of the SmaI/XbaI opened pSV-Sport-Rluc plasmid. The intercistronic region between rLUC and LUCm was verified by sequence analysis at BCCM/GeneCorner. Name mentioned in Cornelis et al. (2005) is Di-pRF. |
| EMBL Accession number: | LT726984.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/01/2007 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AflIII, AvaII, HincII, KpnI and SmaI/XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Y. Bruynooghe(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Cornelis et al., Nucleic Acids Res. 33 (2005), 3095-3108 [PMID: 15928332] |
| Related plasmid reference: | Cienikova et al., RNA 21 (2015), 1931-1942 [PMID: 26370582] [DOI: 10.1261/rna.052373.115] Sherf et al., Promega Notes Magazine 49 (1994), 14-21 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV-Sport-di-RF (LMBP 5253) is available at BCCM/GeneCorner. This plasmid was deposited by Y. Bruynooghe and Prof. Dr R. Beyaert and was published in Cornelis et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.