Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23IVNAm2 (LMBP 3747)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3747.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA); mutated sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23IVNAm1 |
| Further information: | The plasmid was constructed by introducing a StuI site in codons 78 and 79 of the IVNA coding sequence of pSV23IVNAm1, using the site-specific mutagenesis protocol described in Morinaga et al. (1984). This plasmid is useful for isolation of the head region of mature neuraminidase cDNA as a StuI/HindIII fragment. The introduction of the StuI site gave rise to a C78R mutation, and a silent mutation in codon 79. The IVNA coding sequence also includes C42S, D43M and S44A mutations. The nucleotide sequence of wild type IVNA corresponds with the EMBL Nucleotide Sequence Database accession number CY015510.1. Other name of the plasmid is pSV23IVdNAN. |
| EMBL Accession number: | CY015510.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/06/2000 |
| Sequence detail: | Nucleotide sequence around the introduced StuI site compared to the wild type IVNA coding sequence:
78 79
mutated IVNA: 5' GAA.AAA.GAG.ATA.AGG.CCT.AAA.TTA.GTG.GAA.TAC 3'
Glu Lys Glu Ile Arg Pro Lys Leu Val Glu Tyr
StuI
78 79
wt IVNA: 5' GAA.AAA.GAG.ATA.TGC.CCC.AAA.TTA.GTG.GAA.TAC 3'
Glu Lys Glu Ile Cys Pro Lys Leu Val Glu Tyr
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Xavier Saelens(1) (2). (1) Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Wim Martinet (1997) |
| Related plasmid reference: | Morinaga et al., Biotechnology 2 (1984), 636-639 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23IVNAm2 (LMBP 3747) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Xavier Saelens and was published in Wim Martinet, 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.