Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23IVNAm9 (LMBP 3734)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3734.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA); mutated sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23IVNAm8 |
| Further information: | The plasmid was constructed by changing 3 codons to glycine or alanine codons in the IVNA coding sequence of pSV23IVNAm8, using the site-specific mutagenesis protocol described in Morinaga et al. (1984). This plasmid was constructed to investigate the protective potential of neuraminidase mutants towards different influenza strains. The IVNA coding sequence includes the following mutations: C42S, D43M, S44A, C78R, a silent mutation in codon 79, D147G, T153G, a silent mutation in codon 154, H155G, a silent mutation in codon 196, Y197A, K199G, K328A, N329G, R331A, S334G, T336A, N339G, K344A, a silent mutation in codon 345, N346A, H347A, S367A, E368A, D369G, S400A, A401G, R403G, E431G, D463G and L466G. The nucleotide sequence of wild type IVNA corresponds with the EMBL Nucleotide Sequence Database accession number CY015510.1. Other name of the plasmid is pSV23IVdNAN8. |
| EMBL Accession number: | CY015510.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/07/2000 |
| Sequence detail: | Nucleotide sequence of the mutator oligonucleotide compared to the wild type IVNA coding sequence:
147 153 154 155
mutated IVNA 5' CAT.TCA.AAT.GGC.ACA.ATA.CAT.GAT.AGA.GGG.CCC.GGT.CGA.ACC.C 3'
His Ser Asn Gly Thr Ile His Asp Arg Gly Pro Gly Arg Thr
ApaI
147 153 154 155
wt IVNA 5' CAT.TCA.AAT.GAC.ACA.ATA.CAT.GAT.AGA.ACC.CCT.CAT.CGA.ACC.C 3'
His Ser Asn Asp Thr Ile His Asp Arg Thr Pro His Arg Thr
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Xavier Saelens(1) (2). (1) Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Wim Martinet (1997) |
| Related plasmid reference: | Morinaga et al., Biotechnology 2 (1984), 636-639 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23IVNAm9 (LMBP 3734) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Xavier Saelens and was published in Wim Martinet, 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.