Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SE6Hm (LMBP 2153)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2153.gb
(View with Genome Compiler) p2153.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; heavy chain (E6H), mutated sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23SE6H |
| Further information: | This plasmid was derived from pSV23SE6H by introducing a unique KpnI site in front of the start of the mature E6H gene via site-specific mutagenesis. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727624.1. Other names of the plasmid are pSV23SE6H2 and pSV23SE6Hm1. |
| EMBL Accession number: | LT727624.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/04/1990 |
| Sequence detail: | Nucleotide sequence after site-specific mutagenesis:
pSV23SE6H pSV23SE6Hm
Leader | VH Leader | VH
| |
5' GTC CAA TCC | CAG GTT CAG 3' 5' GTC CGG TAC | CAG GTT CAG 3'
Val Gln Ser | Gln Val Gln ** * |
Val Arg Tyr | Gln Val Gln
KpnI
*: Mutated nucleotide. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, BglI/XmnI, ClaI/RsaI, EcoRI, KpnI/SacI, NcoI/XbaI, SalI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SE6Hm (LMBP 2153) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.