Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SHIL4 (LMBP 1922)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p1922.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human interleukin 4 cDNA (IL4, IL-4, BCGF-1, BCGF1, BSF1, GeneID 3565) |
Promoter: | Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pSV23S; pSP65HIL4 |
Further information: | The plasmid was constructed as follows: pSV23S was opened at the unique HindIII site, filled in with Klenow DNA polymerase and redigested with XbaI; then the hIL4 cDNA gene was inserted as a XbaI-XmnI fragment of pSP65HIL4. The plasmid contains the SV40 small t-antigen splicing signal. |
EMBL Accession number: | - |
Latest sequence update: | 16/02/1989 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr W. Fiers(1) (2). It was constructed by B. Jayaram(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | PhD thesis Danny Huylebroeck (1985) |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SHIL4 (LMBP 1922) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr W. Fiers and was published in Danny Huylebroeck, 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.