Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV23SIVNAmTNF (LMBP 3410)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3410.gb
(View with Genome Compiler) p3410.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Influenza A virus (A/Victoria/3/1975(H3N2)) neuraminidase cDNA (NA, IVNA); N-terminal anchor Mouse tumor necrosis factor cDNA (Tnf, TNF); mature sequence |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV23SmTNFm3; pPLaIVNA |
| Further information: | This plasmid was constructed as follows: the small HindIII-FnuDII fragment of pPLaIVNA (containing the first 45 codons of Influenza A/Victoria/3/75 neuraminidase gene) was isolated, provided with a Gly-Ser-Ser, BglII linker, and inserted between the HindIII and BglII sites of pSV23SmTNFm3. This results in the fusion of the neuraminidase anchor to mature mTNF. The plasmid contains the SV40 small t-antigen splicing signal. Restriction enzyme digestions revealed that approx. 60 nucleotides are missing from the 3' untranslated region of the mTNF gene, including the 3'-terminal (supposedly restored) EcoRI site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727483.1. Other name of the plasmid is pSV23SNA.TNF. |
| EMBL Accession number: | M11731, view at EMBL, GenBank, DDBJ LT727483.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/08/1996 |
| Sequence detail: | Fusion region between the A/Victoria/3/75 neuraminidase anchor and
mature, murine TNF:
neuraminidase | | mature mTNF
44 45 | | 1 2 3 4 5 6
5' ...CGA.TCC.CCC.GGG.TCT.AGT.CTC.AGA.TCT.TCT.TCT.CAA... 3'
Gly Ser Ser
BglII
linker sequence : 5' GG.TCT.AGT.CTC.A 3'
3' CC.AGA.TCA.GAG.TCT.AG 5'
BglII |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI/HaeII, BamHI, Bgll/XmnI, Bglll/HindIII, EcoRI/PstI and NcoI/SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Decoster(1)(2) and Prof. Dr W. Fiers(1)(2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV23SIVNAmTNF (LMBP 3410) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Decoster and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.