Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV2Neo (LMBP 3927)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3927.gb
(View with Genome Compiler) p3927.txt p3927.pdf |
| Sequence analysis results Genecorner: |
NGS: b12-gc-dec2018.fasta |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pBR322 |
| Further information: | This shuttle plasmid was designed for efficient cotransfection and expression of non-selected genes. A wide variety of mammalian cells can be used, since the Tn5 neomycin resistance gene provides a marker for dominant selection. The nucleotide sequence of the vector corresponds with the EMBL Nucleotide Sequence Database accession number U02434.1. |
| EMBL Accession number: | U02434.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/03/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AflIII, ApaLI, EcoRI and HindIII. This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Berg(1). (1) Stanford University Medical Center, USA |
| Plasmid reference: | Southern et al., J. Mol. Appl. Genet. 1 (1982), 327-341 [PMID: 6286831] |
| Related plasmid reference: | Rothstein et al., Cold Spring Harb. Symp. Quant. Biol. 45 (1981), 99-105 [PMID: 6271497] Beck et al., Gene 19 (1982), 327-336 [PMID: 6295884] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | LMBP 01991 |
Refer in your Materials and Methods: |
pSV2Neo (LMBP 3927) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Berg and was published in Southern et al., 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.