Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV51B1sFv (LMBP 3608)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3608.gb
(View with Genome Compiler) p3608.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-BCL1 monoclonal antibody IgG1 cDNA; signal sequence (B1H) Mouse anti-BCL1 monoclonal antibody IgG1 cDNA; single chain Fv sequence (B1sFv) Histidine tag (His-tag); C-terminal |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Phage λ T0 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV51B1sFvHG3f; pQEB1sFv |
| Further information: | The plasmid was constructed as follows: pSV51B1sFvHG3f was cut with BspMII/EcoRV (partial BspMII digestion at nucleotide position 4651), and also cut, in a second reaction, with EcoRV/XbaI. 2) The resulting 1062 bp BspMII/EcoRV fragment was then ligated to the 7889 bp EcoRV/XbaI fragment. 3) The 749 bp NheI/BspMII fragment of pQEB1sFv could then be inserted. As a result, the hinge region and CH2 and CH3 domains of the human immunoglobulin γ3 heavy chain (HG3f) were eliminated. The C-terminal polyhistidine tag permits detection and purification of the recombinant B1sFv protein. After transient expression in COS-1 cells, the B1sFv protein was secreted into the culture medium. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727363.1. |
| EMBL Accession number: | LT727363.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/08/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BstXI, EcoRI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2) and Prof. Dr W. fiers(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV51B1sFv (LMBP 3608) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and Prof. Dr W. fiers . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.