Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV57 (LMBP 1835)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psv57.gb
(View with Genome Compiler) psv57.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV54; pPLa83 |
| Further information: | The plasmid was constructed by inserting the BamHI fragment from pPLa83 containing the lac operator, in the unique BamHI site of pSV54. The orientation of the inserted fragment is unknown. See also pSV54 and pSV529 for basic constructions and remarks. Because of the presence of the Lac operator, Lac+ E. coli host cells turn blue on X-Gal. Other name of the plasmid is pSV533. |
| EMBL Accession number: | - |
| Latest sequence update: | 28/11/1989 |
| Sequence detail: | Nucleotide sequence of the inserted BamHI fragment (orientation unknown):
5' GGATCCGGAATT lac operator GAATTCCGGATCCGGA 3'
BamHI EcoRI EcoRI BamHI
BspMII BspMII
BspMII |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, KpnI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Tavernier(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Jan Tavernier |
| Related plasmid reference: | Huylebroeck et al., Gene 66 (1988), 163-181 [PMID: 2844629] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV57 (LMBP 1835) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr W. Min Jou and was published in Jan Tavernier, . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.