Last data update: 24 January 2024 16:39 CET
Plasmid name: pT2-SVT (LMBP 9411)
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| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: | p9411.gb (View with Genome Compiler) |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Simian virus 40 large T-antigen cDNA (SV40gp6, GeneID 29031019) Optimized hyperactive sleeping beauty transposon arms |
| Promoter: | Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pT2/BH; pMaSVT |
| Further information: | The plasmid was constructed by cloning the SV40 large T-antigen from pMaSVT into the EcoRI/XbaI opened pT2/BH vector. This plasmid enables easy immortalization of almost any cell by co-transfection of pT2-SVT and Sleeping Beauty transposase (SB100X). This is far more efficient than transfection of linearized pMaSVT. From a biosafety perspective, it is still as safe as any normal plasmid, in contrast to using the SV40 large T-antigen in for example a lentivirus, which carries a significant biosafety hazard. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/04/2017 |
| Authenticity test: | Restriction enzyme pattern analysed at BCCM/GeneCorner: BamHI, BglI/BglII, PstI and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Ochmann et al., Viruses 13 (2021), 76 [PMID: 33429848] [DOI: https://doi.org/10.3390/v13010076] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pT2-SVT (LMBP 9411) is available at BCCM/GeneCorner. The plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.