Last data update: 24 January 2024 16:39 CET
Plasmid name: pTOPTK-iGFP (LMBP 6847)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6847.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Discosoma sp. red fluorescent (tetrameric) protein (DsRed) |
| Promoter: | Human E-cadherin promoter (hE-cad) Herpes simplex virus (HSV) thymidine kinase promoter (TK) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter E1b basal promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Further information: | Other name of the plasmid is TOPtkGAL4VP16GR-UASeGFP+hECprDsRed. |
| EMBL Accession number: | - |
| Latest sequence update: | 02/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. Vleminckx(1) (2). It was constructed by Dr T. Denayer(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Denayer et al., FEBS Lett. 580 (2006), 393-398 [PMID: 16376877] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTOPTK-iGFP (LMBP 6847) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. Vleminckx and was published in Denayer et al., 2006. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.