Last data update: 24 January 2024 16:39 CET
Plasmid name: pTetO7TATA-dCas9-Mxi1-HIS3-CNE1gRNA3 (LMBP 11110)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11110.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Guide RNA targeting Saccharomyces cerevisiae calnexin (CNE1, GeneID 851241) Streptococcus pyogenes CRISPR associated protein 9 cDNA (Cas9); human-codon-optimised MAX interactor 1 dimerization protein cDNA (Mxi1) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE); minimal promoter with tet operator (CMVT) Saccharomyces cerevisiae SNR52 RNA polymerase III promoter (SNR52) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae suppressor SUP4 tRNA terminator (SUP4) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae HIS3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 |
| Host range: | Escherichia coli Saccharomyces cerevisiae; his3(-) |
| Parental clone: | pTetO7TATA-dCas9-Mxi1-HIS3 |
| Further information: | The plasmid was constructed by cloning a 20 bp gRNA targeting S. cerevisiae CNE1 into the doxicyclin repressible pTetO7TATA-dCas9-Mxi1-HIS3 vector. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/09/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1) (2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Morgane Boone (2017) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTetO7TATA-dCas9-Mxi1-HIS3-CNE1gRNA3 (LMBP 11110) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Morgane Boone, 2017. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.